![]() The sssvLAMP method was more efficient in detecting 6.50 × 10 1–6.45 × 10 4-fold low number of V. Positive results were simply read by the naked eye via color change (from orange to light green) under the visible light, or by the production of green fluorescence under the UV light (260 nm). The optimal conditions of the sssvLAMP method was determined, and one-step sssvLAMP reaction was performed at 65☌ for 40 min. Three pairs of oligonucleotide primers (inner, outer, and loop primers) were designed and or synthesized to target each of these genes. In this study, a simple, sensitive, specific, and visualized method was developed based on loop-mediated isothermal amplification (LAMP) (designated sssvLAMP) to detect virulence-associated ( ctxA, tcpA, hapA, mshA, pilA, and tlh) and species-specific ( lolB) genes of V. cholerae contamination in drinking water and aquatic products is imperative for assuring food safety. It can cause human cholera that is still pandemic in developing nations. ![]() Vibrio cholerae is a leading waterborne pathogenic bacterium worldwide.
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